FUNCTIONAL INTERACTION OF THE CARBOXYLIC-ACID GROUP OF AGONISTS AND THE ARGININE RESIDUE OF THE 7TH TRANSMEMBRANE DOMAIN OF PROSTAGLANDIN-ERECEPTOR EP3 SUBTYPE

Prostaglandin (PG) E(2) binds to PGE receptor EP3 subtype and induces G(i) activity. To assess the role of the interaction of the carboxylic acid group of agonists and its putative binding site, Arg-309 in the seventh transmembrane domain of EP3 alpha receptor, in receptor activa tion, we have mutated the positively charged Arg-309 to the polar but uncharged Gln (EP3 alpha-R309Q) and Asn (EP3 alpha-R309N), and to the non-polar Leu (EP3 alpha-R309L). Wild-type, EP3 alpha-R309Q and EP3 al pha-R309N receptors showed high-affinity binding for PGE(2), but the E P3 alpha-R309L receptor showed very-low-affinity binding. Guanosine 5' -[gamma-thio]triphosphate increased the PGE(2) binding to the wild-typ e receptor, decreased the binding to EP3 alpha-R309Q and EP3 alpha-R30 9N receptors, but did not affect that to the EP3 alpha-R309L receptor. Furthermore we examined the G(i) activities of two types of EP3 agoni st, TEI-3356 with a negatively charged carboxylic acid, and TEI-4343, a methyl ester of TEI-3356 with an uncharged but polar group, towards those receptors. Both agonists inhibited the forskolin-stimulated cAMP formation in wild-type, EP3 alpha-R309Q and EP3 alpha-R309N receptors in the same concentration-dependent manner, but the agonists showed a very low inhibition of EP3 alpha-R309L receptor. These findings demon strate that the hydrogen-bonding interaction of EP3 agonists and resid ue 309 in the seventh transmembrane domain of the EP3 alpha receptor i s sufficient for the functional activation of the EP3 alpha receptor.