ISOLATION OF THE YEAST HISTONE OCTAMER

Authors
LORCH Y KORNBERG RD
Citation
Y. Lorch et Rd. Kornberg, ISOLATION OF THE YEAST HISTONE OCTAMER, Proceedings of the National Academy of Sciences of the United Statesof America, 91(23), 1994, pp. 11032-11034
Citations number
28
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
91
Issue
23
Year of publication
1994
Pages
11032 - 11034
Database
ISI
SICI code
0027-8424(1994)91:23<11032:IOTYHO>2.0.ZU;2-#
Abstract
Procedures for the extraction and purification of the yeast histone oc tamer are described. Either mechanical disruption, yielding chromatin fragments, or spheroplast formation with subsequent nuclear isolation was employed. A hexahistidine tag was inserted in the N-terminal regio n of histone H2B, permitting resolution of the histone octamer from hi gh-salt extracts of nuclei or chromatin to near homogeneity. The histo ne octamer purified in this way was fully active in reconstitution of nucleosomes.