IDENTIFICATION OF GENES INVOLVED IN DNA-REPLICATION OF THE AUTOGRAPHA-CALIFORNICA BACULOVIRUS

Authors
KOOL M AHRENS CH GOLDBACH RW ROHRMANN GF VLAK JM
Citation
M. Kool et al., IDENTIFICATION OF GENES INVOLVED IN DNA-REPLICATION OF THE AUTOGRAPHA-CALIFORNICA BACULOVIRUS, Proceedings of the National Academy of Sciences of the United Statesof America, 91(23), 1994, pp. 11212-11216
Citations number
57
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
91
Issue
23
Year of publication
1994
Pages
11212 - 11216
Database
ISI
SICI code
0027-8424(1994)91:23<11212:IOGIID>2.0.ZU;2-M
Abstract
By use of a transient replication assay, nine genes involved in DNA re plication were identified in the genome of the Autographa californica baculovirus. Six genes encoding helicase, DNA polymerase, IE-1, LEF-I, LEF-2, and LEF-3 are essential for DNA replication while three genes encoding P35, IE-2, and PE38 stimulate DNA replication. No stimulation by the AcMNPV pcna gene, encoding a protein with sequence homology to proliferating-cell nuclear antigen, was observed. A pattern of amino acids found in a number of single-stranded-DNA-binding proteins was id entified in the carboxyl-terminal region of IE-1.