A ZYMOGRAM METHOD TO DETECT ENDOGLUCANASES FROM BACILLUS-SUBTILIS, MYROTHECIUM-VERRUCARIA AND TRICHODERMA-REESEI

Authors
HOLT SM HARTMAN PA
Citation
Sm. Holt et Pa. Hartman, A ZYMOGRAM METHOD TO DETECT ENDOGLUCANASES FROM BACILLUS-SUBTILIS, MYROTHECIUM-VERRUCARIA AND TRICHODERMA-REESEI, Journal of industrial microbiology, 13(1), 1994, pp. 2-4
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Journal of industrial microbiology → ACNP
ISSN journal
01694146
Volume
13
Issue
1
Year of publication
1994
Pages
2 - 4
Database
ISI
SICI code
0169-4146(1994)13:1<2:AZMTDE>2.0.ZU;2-V
Abstract
A purified endoglucanase (Eg) from Bacillus subtilis and crude Eg prep arations from Myrothecium verrucaria and Trichoderma reesei were denat ured by heat in the presence of SDS and separated by using SDS polyacr ylamide gel electrophoresis. Eg zymogram activity bands were detected directly in carboxymethylcellulose-laden separating gels after enzyme renaturation. Denaturing zymogram analysis was a simple method for the separation and detection of Egs.