TRANSFORMATION OF ASPERGILLUS-PARASITICUS USING AUTONOMOUSLY REPLICATING PLASMIDS FROM ASPERGILLUS-NIDULANS

Authors
MORENO MA PASCUAL C GIBELLO A FERRER S BOS CJ DEBETS AJM SUAREZ G
Citation
Ma. Moreno et al., TRANSFORMATION OF ASPERGILLUS-PARASITICUS USING AUTONOMOUSLY REPLICATING PLASMIDS FROM ASPERGILLUS-NIDULANS, FEMS microbiology letters, 124(1), 1994, pp. 35-41
Citations number
25
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
124
Issue
1
Year of publication
1994
Pages
35 - 41
Database
ISI
SICI code
0378-1097(1994)124:1<35:TOAUAR>2.0.ZU;2-G
Abstract
A genetic transformation system for the aflatoxin-producing fungus Asp ergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5x10(2) to 2.5x10(4) transformants per 10(6) viable protoplasts and mu g DNA. The stability of the plasmi ds in the transformants was also studied. This transformation system o ffers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants.