CATALYTIC PROPERTIES OF SOYBEAN LIPOXYGEN ASE, IMMOBILIZED ON MODIFIED SILICA

Catalytic properties of soybean lipoxygenase immobilized on silica hav e been studied. Lipoxygenase had the highest activity in the case of c ovalent immobilization on aminosilica activated by glutaraldehyde or o n carboxysilica activated by N-hydroxysuccinimide and after physical s orption on aminosilica. Kinetic properties of immobilized lipoxygenase in the reaction of oxygenation of linoleic acid proved to be as follo ws optimal pH 9.0; observed K(m) 0.104 +/- 0.20 mM. The observed resid ual activity of the immobilized enzyme was 4-16%. The immobilized lipo xygenase has been used for the synthesis of 13(S)-hydroperoxide of lin oleic acid with a 96-98% yield. The product's structure was studied by UV and IR spectrometry, HPLC, TLC and polarimetry.