CATALYTIC PROPERTIES OF SOYBEAN LIPOXYGEN ASE, IMMOBILIZED ON MODIFIED SILICA

Citation
Ia. Butovich et al., CATALYTIC PROPERTIES OF SOYBEAN LIPOXYGEN ASE, IMMOBILIZED ON MODIFIED SILICA, Bioorganiceskaa himia, 20(10), 1994, pp. 1095-1100
Citations number
22
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
ISSN journal
01323423
Volume
20
Issue
10
Year of publication
1994
Pages
1095 - 1100
Database
ISI
SICI code
0132-3423(1994)20:10<1095:CPOSLA>2.0.ZU;2-P
Abstract
Catalytic properties of soybean lipoxygenase immobilized on silica hav e been studied. Lipoxygenase had the highest activity in the case of c ovalent immobilization on aminosilica activated by glutaraldehyde or o n carboxysilica activated by N-hydroxysuccinimide and after physical s orption on aminosilica. Kinetic properties of immobilized lipoxygenase in the reaction of oxygenation of linoleic acid proved to be as follo ws optimal pH 9.0; observed K(m) 0.104 +/- 0.20 mM. The observed resid ual activity of the immobilized enzyme was 4-16%. The immobilized lipo xygenase has been used for the synthesis of 13(S)-hydroperoxide of lin oleic acid with a 96-98% yield. The product's structure was studied by UV and IR spectrometry, HPLC, TLC and polarimetry.