LOSS OF APC(+) IN INTESTINAL ADENOMAS FROM MIN MICE

Allelic loss at the Apc locus in spontaneously occurring intestinal ad enomas from mice heterozygous for the Apc(Min) nonsense mutation was a nalyzed using a site-specific quantitative polymerase chain reaction a ssay. All 97 of the intestinal adenomas analyzed showed extensive loss of the wild-type Apc (Apc(+)) allele. Quantitative polymerase chain r eaction analysis of loci linked to Apc indicated loss of the chromosom e carrying Apc(+) Only one copy of the homologue carrying Apc(Min) rem ained in the intestinal adenomas. Possible reasons for the difference in the mechanism of Apc(+) loss between human and Min mouse intestinal adenomas are discussed.