ANALYSIS OF DOUBLE-STRANDED OLIGONUCLEOTIDES BY ELECTROSPRAY MASS-SPECTROMETRY

Authors
BAYER E BAUER T SCHMEER K BLEICHER K MALER M GAUS HJ
Citation
E. Bayer et al., ANALYSIS OF DOUBLE-STRANDED OLIGONUCLEOTIDES BY ELECTROSPRAY MASS-SPECTROMETRY, Analytical chemistry, 66(22), 1994, pp. 3858-3863
Citations number
30
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry → ACNP
ISSN journal
00032700
Volume
66
Issue
22
Year of publication
1994
Pages
3858 - 3863
Database
ISI
SICI code
0003-2700(1994)66:22<3858:AODOBE>2.0.ZU;2-X
Abstract
Double-stranded oligonucleotides of different lengths and chemical mod ification have been analyzed by ion spray mass spectrometry. The non-c ovalent-bonded duplexes can be detected. Therefore, ion spray mass spe ctrometry is a useful method for investigation of hybridizations of na tural and chemically modified oligonucleotides. Since the exact mass o f the double strand can be detected, this method can distinguish betwe en specific and nonspecific interaction.