SEPARATION OF FRAGMENTS UP TO 570 BASES IN LENGTH BY USE OF 6-PERCENTT-NON-CROSS-LINKED POLYACRYLAMIDE FOR DNA-SEQUENCING IN CAPILLARY ELECTROPHORESIS

Authors
BEST N ARRIAGA E CHEN DY DOVICHI NJ
Citation
N. Best et al., SEPARATION OF FRAGMENTS UP TO 570 BASES IN LENGTH BY USE OF 6-PERCENTT-NON-CROSS-LINKED POLYACRYLAMIDE FOR DNA-SEQUENCING IN CAPILLARY ELECTROPHORESIS, Analytical chemistry, 66(22), 1994, pp. 4063-4067
Citations number
49
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry → ACNP
ISSN journal
00032700
Volume
66
Issue
22
Year of publication
1994
Pages
4063 - 4067
Database
ISI
SICI code
0003-2700(1994)66:22<4063:SOFUT5>2.0.ZU;2-B
Abstract
Non-cross-linked polyacrylamide is a very convenient medium for the se paration of DNA sequencing fragments in capillary electrophoresis. We demonstrate DNA sequencing with this matrix at an electric field of 20 0 V/cm and at room temperature. Resolution is observed to decrease exp onentially with fragment length. Fragments 570 bases in length generat e a resolution of 0.5, which is adequate for sequence identification.