CONSERVATION OF THE CYTOTOXIN-ASSOCIATED (CAGA) GENE OF HELICOBACTER-PYLORI AND INVESTIGATION OF ASSOCIATION WITH VACUOLATING-CYTOTOXIN ACTIVITY AND GASTRODUODENAL DISEASE
Rj. Owen et al., CONSERVATION OF THE CYTOTOXIN-ASSOCIATED (CAGA) GENE OF HELICOBACTER-PYLORI AND INVESTIGATION OF ASSOCIATION WITH VACUOLATING-CYTOTOXIN ACTIVITY AND GASTRODUODENAL DISEASE, FEMS immunology and medical microbiology, 9(4), 1994, pp. 307-315
Polymerase chain reaction (PCR) amplification and DNA hybridization an
alyses were used to test for the presence of the cytotoxin-associated
(cagA) gene in 108 strains of Helicobacter pylori. Fifty-two geographi
cally diverse strains of known vacuolating cytotoxin activity, and 56
recent UK clinical isolates from patients with duodenal ulceration (n
= 28) and from healthy individuals who were endoscopically normal (n =
28) were studied. Overall, cagA was detected by PCR in 74 (69%) strai
ns and DNA hybridization provided evidence of gene homologues in a fur
ther eight strains. For 95% of the cytotoxin-producing strains and 46%
of the non-cytotoxin producing strains, there was a close association
either with presence or absence of cagA. At the genomic level, Southe
rn blot DNA hybridization showed that cagA was probably present in a s
ingle copy in most of the H pylori tested, and that HaeIII restriction
site variation within and around the gene provided additional markers
of diversity for the species. As 40% of the cagA containing strains d
id not produce an active cytotoxin, and no significant association bet
ween cagA presence and DU-disease was observed, we concluded that the
presence of the cagA gene in H. pylori could not be used as a single r
eliable predictor of higher risk patients.