Cytotoxic monoclonal antibody IVA 44 was generated after the intraperi
toneal immunization with peripheral blood mononuclear (PBM) cells and
the boost by the intrasplenic inoculation of skin graft. The detected
membrane antigen isolated by immunoprecipitation appears to be compose
d of two subunits characteristic for the MHC class I molecules. The an
tibody IVA 44 exhibited a different reactivity: it recognized the BoLA
A14 (A8) specificity in animals typed in the Fifth BoLA workshop, whi
le it reacted with all As positive animals including subtypes A14 and
A15 in Czech and Slovak cattle. It is concluded that mAb IVA 44 might
detect the broad subtype of As covering A14 and certain A15 split(s).
The diverse A15 reactivity of this mAb in the workshop and our populat
ion could be explained by the different occurrence of A15 splits in ba
th populations.