A RAPID PROTOCOL FOR SEXING CHICK-EMBRYOS (GALLUS-G-DOMESTICUS)

Authors
CLINTON M
Citation
M. Clinton, A RAPID PROTOCOL FOR SEXING CHICK-EMBRYOS (GALLUS-G-DOMESTICUS), Animal genetics, 25(5), 1994, pp. 361-362
Citations number
5
Categorie Soggetti
Genetics & Heredity","Veterinary Sciences
Journal title
Animal genetics → ACNP
ISSN journal
02689146
Volume
25
Issue
5
Year of publication
1994
Pages
361 - 362
Database
ISI
SICI code
0268-9146(1994)25:5<361:ARPFSC>2.0.ZU;2-4
Abstract
A method for establishing the sex of chick embryos before the appearan ce or morphological differences between males and females has been dev eloped. DNA was isolated from 5-7-day-old embryos by proteinase K dige stion and subjected to polymerase chain reaction (PCR) amplification w ith W-chromosome specific primers. Sexing can be achieved within 1 day using as little as 1 ng template DNA.