SEPARATION OF CHLAMYDOSPOROL EPIMERS BY REVERSED-PHASE HPLC USING COMMERCIAL SOLVENT OPTIMIZATION SOFTWARE

Authors
SOLFRIZZO M FORBESSMITH M STRANGE RN VISCONTI A
Citation
M. Solfrizzo et al., SEPARATION OF CHLAMYDOSPOROL EPIMERS BY REVERSED-PHASE HPLC USING COMMERCIAL SOLVENT OPTIMIZATION SOFTWARE, Chromatographia, 39(7-8), 1994, pp. 443-447
Citations number
12
Categorie Soggetti
Chemistry Analytical
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
39
Issue
7-8
Year of publication
1994
Pages
443 - 447
Database
ISI
SICI code
0009-5893(1994)39:7-8<443:SOCEBR>2.0.ZU;2-B
Abstract
Two epimers of the mycotoxin chlamydosporol were separated by HPLC on an RP-18 column using a quaternary mobile phase consisting of water (7 9.1 %), methanol (10.0 %), acetonitrile (10.4 %) and tetrahydrofuran ( 0.5 %), with a flow rate of 1 ml min(-1). This optimal composition of mobile phase, with which the resolution value for the two epimers (1 a nd 2) was 2.73 with retention times of 5.88 and 7.12 min, respectively , Was achieved by the application of Philips Solvent Optimisation Soft ware PU 6100. The presence of free silanols on the stationary phase wa s shown to be an essential requirement for the separation of the chlam ydosporol epimers.