M. Solfrizzo et al., SEPARATION OF CHLAMYDOSPOROL EPIMERS BY REVERSED-PHASE HPLC USING COMMERCIAL SOLVENT OPTIMIZATION SOFTWARE, Chromatographia, 39(7-8), 1994, pp. 443-447
Two epimers of the mycotoxin chlamydosporol were separated by HPLC on
an RP-18 column using a quaternary mobile phase consisting of water (7
9.1 %), methanol (10.0 %), acetonitrile (10.4 %) and tetrahydrofuran (
0.5 %), with a flow rate of 1 ml min(-1). This optimal composition of
mobile phase, with which the resolution value for the two epimers (1 a
nd 2) was 2.73 with retention times of 5.88 and 7.12 min, respectively
, Was achieved by the application of Philips Solvent Optimisation Soft
ware PU 6100. The presence of free silanols on the stationary phase wa
s shown to be an essential requirement for the separation of the chlam
ydosporol epimers.