HPLC FRACTIONATION OF RAT-LIVER TRANSFER-RNA - ISOLATION OF TRNA(GLU), TRNA(GLN), TRNA(PHE), AND TRNA(THR)

Authors
KANDUC D BRACALELLO A MOSAVI A
Citation
D. Kanduc et al., HPLC FRACTIONATION OF RAT-LIVER TRANSFER-RNA - ISOLATION OF TRNA(GLU), TRNA(GLN), TRNA(PHE), AND TRNA(THR), Biochemical archives, 10(4), 1994, pp. 243-248
Citations number
15
Categorie Soggetti
Biology
Journal title
Biochemical archives → ACNP
ISSN journal
07495331
Volume
10
Issue
4
Year of publication
1994
Pages
243 - 248
Database
ISI
SICI code
0749-5331(1994)10:4<243:HFORT->2.0.ZU;2-F
Abstract
tRNA from rat liver has been fractionated by h.p.l.c. using a gradient of acetonitrile/ammonium acetate. Peak assignment and relative concen trations of iso-tRNAs for phenylalanine, glutamic acid, glutamine and threonine have been established. The relative concentrations of these tRNA species, expressed as area peak percentages, have been found to c orrespond well with codon usage for human proteins. The data confirm t he reliability of this technique as simple method for fractionating co ntemporaneously a variety of tRNAs on analytical scale.