CHIRAL SEPARATION OF DRUG ENANTIOMERS BY CAPILLARY ELECTROPHORESIS USING SUCCINYL-BETA-CYCLODEXTRIN

A capillary electrophoretic method for the enantiomeric separation of 11 drugs was developed using an uncoated fused-silica capillary and su ccinyl beta-cyclodextrin as a chiral additive. The effect of the pH of the background electrolyte on selectivity and resolution was studied in the range pH 3.3-9.3. Best results were obtained in a neutral mediu m. Generally, the presence of a hydroxy group at the chiral C-atom of the analyte seems to be essential because similar compounds without a hydroxy group at the chiral centre did not show chiral resolution. In addition to the enantioselective inclusion into the chiral cavity, hyd rogen bondings and formation of ion pairs between the negatively charg ed selector and cationic analytes can be assumed as mechanisms.