S. Hanem et al., INCREASED INTRACELLULAR POTASSIUM AND WATER CONTENTS IN RAT-HEART AFTER ALPHA-1-ADRENOCEPTOR STIMULATION, Pharmacology & toxicology, 75(5), 1994, pp. 294-301
Potassium accumulation in rat heart after alpha-1-adrenoceptor stimula
tion has previously been reported from indirect measurements. Here we
present data on intracellular potassium content measured directly in t
he heart. Isolated rat hearts perfused in a non-recirculating system w
ere exposed to alpha-1-adrenoceptor stimulation (5 X 10(-5) mol/l phen
ylephrine in the presence of 10(-6) mol/l timolol). C-14-Sucrose was u
sed to estimate the extracellular space. From heart homogenates intrac
ellular potassium, magnesium and cellular water contents were determin
ed and the ion concentrations calculated accordingly. The intracellula
r magnesium content remained unchanged during all experimental conditi
ons. alpha-1-Adrenoceptor stimulation evoked an increase in potassium
content by 9% (4, 14; 95% confidence interval (CI), P=0.0006). Due to
an observed increase in intracellular water by 17% (9, 26; 95% CI, P=0
.0006), the potassium concentration apparently decreased by 8% (0.3, 1
5; 95% CI, P=0.04). During partial inhibition of the Na+/K+-ATPase by
10(-5) mol/l ouabain, there was an increase in potassium content by 5%
(1, 9; 95% CI, P=0.008). There was, however, no significant increase
in intracellular water in this situation. Calculated intracellular pot
assium concentration showed accordingly a slight increase. The effects
upon potassium and water both in the absence and presence of ouabain
were eliminated by the alpha-1-adrenoceptor blocker prazosin (10(-6) m
ol-l). alpha-1-Adrenoceptor stimulation apparently increased cellular
dry weight by 10% (2, 18; 95% CI, P=0.02). Changes in translocation of
potassium and water must be considered as part of the alpha-1-adrener
gic heart effects.