CONSTRUCTION AND SCREENING OF A RYE DNA LIBRARY FOR RFLP MAPPING

Authors
KORZUN V KARTEL N PLASCHKE J BORNER A
Citation
V. Korzun et al., CONSTRUCTION AND SCREENING OF A RYE DNA LIBRARY FOR RFLP MAPPING, Cereal Research Communications, 22(3), 1994, pp. 151-157
Citations number
NO
Categorie Soggetti
Agriculture
Journal title
Cereal Research Communications → ACNP
ISSN journal
01333720
Volume
22
Issue
3
Year of publication
1994
Pages
151 - 157
Database
ISI
SICI code
0133-3720(1994)22:3<151:CASOAR>2.0.ZU;2-E
Abstract
For the development of saturated linkage maps of plants, it is necessa ry to get as many informative probes as possible. By using, the methyl ation sensitive restriction enzyme PstI, we developed a library of sin gle/low-copy DNA sequences of rye. A total of 180 clones were selected , 12 sequences were located on rye and wheat chromosomes and two of th em were mapped with respect to known RFLP markers on chromosomes 5R an d 7R.