A NEW HPLC METHOD FOR THE QUANTIFICATION OF CARDENOLIDES IN CONVALLARIA-MAJALIS L

Authors
KRENN L SCHLIFELNER L STIMPFL T KOPP B
Citation
L. Krenn et al., A NEW HPLC METHOD FOR THE QUANTIFICATION OF CARDENOLIDES IN CONVALLARIA-MAJALIS L, Die Pharmazie, 51(11), 1996, pp. 906-909
Citations number
11
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
Die Pharmazie → ACNP
ISSN journal
00317144
Volume
51
Issue
11
Year of publication
1996
Pages
906 - 909
Database
ISI
SICI code
0031-7144(1996)51:11<906:ANHMFT>2.0.ZU;2-Q
Abstract
A new RP-HPLC method was developed for the determination of cardenolid es in Convallaria majalis. Hypersil ODS 3 mu m as stationary and aceto nitrile/water (88 + 12) as mobile phase and gradient elution were used for the separation. For the quantification of the total and single am ounts of the cardenolides helveticoside was added as internal standard . The reproducibility and accuracy of the method as well as the natura l variability of the cardenolide complex in Herba Convallariae were de termined by analysis of 13 samples of C.