IMMUNOCYTOCHEMICAL LOCALIZATION OF THE HIGH-MOLECULAR-WEIGHT PROTEASEIN BRAIN

Polyclonal antiserum against a high molecular weight glycosylated prot ease, purified from calf brain cytosol, was raised in rabbit and purif ied by immunoaffinity. The antibody specifically immunoreacted with th e M(r) = 165,000 and 155,000 polypeptides of the protease. Immunocytoc hemical localization data revealed that the protease is localized in t he pyramidal neurons, granular and glial cells of the hippocampus. Mic roscopic analysis of the pyramidal neurons indicates that the protease is present in the cytoplasm and extends to the dendrite and axon. The nuclei of these neurons remain unstained.