INHERITANCE OF POLYMORPHIC MARKERS GENERATED BY DNA AMPLIFICATION FINGERPRINTING AND THEIR USE AS GENETIC-MARKERS IN SOYBEAN

DNA amplification fingerprinting (DAF) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (AFLP) in soybean (Glyci ne max (L.) Merr.). The inheritance of AFLPs was studied using a cross between the ancestral Glycine soja PI468.397 and Glycine max (L.) Mer r. line nts382, F1 and F2 progeny. The amplification reaction was carr ied out with soybean genomic DNA and 8 base long oligonucleotide prime rs. Silver-stained 5% polyacrylamide gels containing 7 M urea detected from 11 to 28 DAF products with primers of varying GC content (rangin g from 50 to 100% GC). Depending on their intensity, AFLPs were classi fied into three classes. DAF profiles were reproducible for different DNA extractions and gels. Forty AFLPs were detected by 26 primers when comparing G. soja and G. max. Most AFLPs were inherited as dominant M endelian markers in F1 and F2 populations. However, abnormal inheritan ce occured with about 25% of polymorphisms. One marker was inherited a s a maternal marker, presumably originating from organelle DNA while a nother showed apparent paternal inheritance. To confirm the nuclear or igin and utility of dominant Mendelian markers, three DAF polymorphism s were mapped using a F11 mapping population of recombinant inbred lin es from soybean cultivars Minsoy x Noir 1. The study showed that DAF-g enerated polymorphic markers occur frequently and reliably, that they are inherited as Mendelian dominant loci and that they can be used in genome mapping.