CHARACTERIZATION OF A PREDOMINANTLY PISTIL-EXPRESSED GENE ENCODING A GAMMA-THIONIN-LIKE PROTEIN OF PETUNIA-INFLATA

Authors
KARUNANANDAA B SINGH A KAO TH
Citation
B. Karunanandaa et al., CHARACTERIZATION OF A PREDOMINANTLY PISTIL-EXPRESSED GENE ENCODING A GAMMA-THIONIN-LIKE PROTEIN OF PETUNIA-INFLATA, Plant molecular biology, 26(1), 1994, pp. 459-464
Citations number
19
Categorie Soggetti
Plant Sciences",Biology
Journal title
Plant molecular biology → ACNP
ISSN journal
01674412
Volume
26
Issue
1
Year of publication
1994
Pages
459 - 464
Database
ISI
SICI code
0167-4412(1994)26:1<459:COAPPG>2.0.ZU;2-F
Abstract
We isolated a cDNA clone from a pistil cDNA library of Petunia inflata which encodes a protein, PPT, with sequence similarity to gamma-thion ins. Characterization of a genomic clone containing a PPT gene reveale d the presence of a single intron. Northern analysis revealed that the PPT gene was predominantly expressed in the pistil during all stages of flower development. Since thionins have been implicated in plant de fense against pathogens, PPT may play a role similar to that of other defense-related proteins found in the pistil, defending the pistil aga inst pathogen infection.