ENZYMATIC TRANSFORMATION OF CEPHALOSPORIN-C TO 7-AMINO-CEPHALOSPORANIC ACID .1. CULTIVATION OF PSEUDOMONAS-SYRINGAE AND PARTIAL-PURIFICATION AND IMMOBILIZATION OF 7-BETA-(4-CARBOXYBUTANAMIDO) CEPHALOSPORANIC ACID ACYLASE

The enzymatic transformation of 7-beta-(4-carboxybutanamido)cephalospo ranic acid (Gl-7-ACA) to 7-amino-cephalosporanic acid (7-ACA) is repor ted. The optimum conditions for cultivation of the producer strain Pse udomonas syringae, as well as the procedures for isolation, purificati on, and immobilization of the enzyme Gl-7-ACA acylase, are described. It is shown that when glutaraldehyde is used for immobilization of thi s enzyme, the yield of immobilization is low. After six hydrolyses of Gl-7-ACA to 7-AGA, the immobilized enzyme activity loss is less than 1 0%.