ENZYMATIC TRANSFORMATION OF CEPHALOSPORIN-C TO 7-AMINO-CEPHALOSPORANIC ACID .2. SINGLE-STEP PROCEDURE USING A COIMMOBILIZED ENZYME-SYSTEM

The enzymatic transformation of cephalosporin C to 7-amino-cephalospor anic acid (7-ACA) using coimmobilized D-aminoacid oxidase (DAAO) and 7 -beta-(4-carboxybutanamido)cephalosporanic acid acylase (Gl-7-ACA acyl ase) is reported, The results from the coimmobilization of the two enz ymes on different carriers and at different ratios of enzyme activitie s are described. When an inhibitor of catalase activity such as NaN3 o r H2O2 is present, the conversion rate to 7-ACA is higher, but more by -products are obtained. An optimum ratio of 60:1 between the enzymatic activities of DRAAO and Gl-7-ACA acylase in the coimmobilized sample at 0.21 U g(-1) Cl-7-ACA acylase activity was determined. The results of using coimmobilized enzymes and of using a mixture of separately im mobilized enzymes in the same process are compared.