P40(MO15) ASSOCIATES WITH A P36 SUBUNIT AND REQUIRES BOTH NUCLEAR TRANSLOCATION AND THR176 PHOSPHORYLATION TO GENERATE CDK-ACTIVATING KINASE-ACTIVITY IN XENOPUS OOCYTES

p40(MO15), cdc2-related protein, is the catalytic subunit of the kinas e (CAK, cdk-activating kinase) responsible for Thr161/Thr160 phosphory lation and activation of cdk1/cdk2. We have found that strong overexpr ession of p40(MO15) only moderately increases CAK activity in Xenopus oocytes, indicating that a regulatory CAK subunit (possibly a cyclin-l ike protein) limits the ability to generate CAK activity in p40(MO15) overexpressing oocytes. This 36 kDa subunit was microsequenced after e xtensive purification of CAK activity. Production of Xenopus CAK activ ity was strongly reduced in enucleated oocytes overexpressing p40(MO15 ) and p40(MO15) shown to contain a nuclear localization signal require d for nuclear translocation and generation of CAK activity. p40(MO15) was found to be phosphorylated on Serl70 and Thr176 by proteolytic deg radation, radiosequencing of tryptic peptides and mutagenesis. Thr176 phosphorylation is required and Serl70 phosphorylation is dispensable for p40(MO15) to generate CAK activity upon association with the 36 kD a regulatory subunit. Finally, Thr176 and Serl70 phosphorylations are not intramolecular autophosphorylation reactions. Taken together, the above results identify protein-protein interactions, nuclear transloca tion and phosphorylation (by an unidentified kinase) as features of p4 0(MO15) that are required for the generation of active CAK.