The application of a diagnostic and genotyping technique based on the
polymerase chain reaction (PCR) to the study of trachoma epidemiology
in the Gambian village of Jali is reported. PCR based on the major out
er membrane protein (MOMP) gene of Chlamydia trachomatis appears to be
more sensitive than either isolation or antigen detection by enzyme i
mmunoassay; it had a specificity of 95% and sensitivity of 51% against
clinical signs. PCR genotyping identified genotypes A and B of Chlamy
dia trachomatis circulating in Jali. Sequencing revealed a Pst1 restri
ction endonuclease site in the amplified MOMP gene of some B strains b
ut not others; Pst1 digestion of the PCR product proved an easy method
of distinguishing these strains. The distribution of serotypes and B
strain variants shows a significant degree of household clustering (p<
0.001). PCR based genotyping combined with strain typing provides a ne
w and powerful epidemiological tool for the study of transmission even
ts in trachoma.