CONTROLLED LYSIS OF ESCHERICHIA-COLI DOUBLE-LYSOGEN BY UV-IRRADIATION

Authors
PARK SS KOO YM
Citation
Ss. Park et Ym. Koo, CONTROLLED LYSIS OF ESCHERICHIA-COLI DOUBLE-LYSOGEN BY UV-IRRADIATION, Biotechnology letters, 16(10), 1994, pp. 1007-1010
Citations number
7
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
16
Issue
10
Year of publication
1994
Pages
1007 - 1010
Database
ISI
SICI code
0141-5492(1994)16:10<1007:CLOEDB>2.0.ZU;2-8
Abstract
Sequential protein production and cell disruption was studied using. E . coli double-lysogen, P90c/(lambda LHL1,phi 434). The induction of th e first prophage lambda HL1 to produce beta-galactosidase by the tempe rature increase was followed by the induction of the second prophage p hi 434 by the W-irradiation to lyze the cell. The optimum operating co nditions for the UV-irradiation were sought to yield the maximum cell lysis.