The activities of poyphenol oxidase, peroxidase, acid phosphatase and
trypsin were measured in the presence of four different water miscible
solvents, acetonitrile; N,N, dimethylformamide; tetrahydrofuran and d
ioxane. Stimulation of enzymatic activities was observed in all the ca
ses at a specific concentration range of cosolvent added which cannot
be correlated either with log P or denaturation capacity. Also, no sig
nificant structural changes were observed by fluorescence studies even
at the cosolvent concentrations where activity increases were signifi
cant.