MODIFICATION OF LIPASE FROM PSEUDOMONAS SP WITH POLY(ACRYLOYLMORPHOLINE) AND STUDY OF ITS CATALYTIC PROPERTIES IN ORGANIC-SOLVENTS

Authors
BOVARA R OTTOLINA G CARREA G FERRUTI P VERONESE FM
Citation
R. Bovara et al., MODIFICATION OF LIPASE FROM PSEUDOMONAS SP WITH POLY(ACRYLOYLMORPHOLINE) AND STUDY OF ITS CATALYTIC PROPERTIES IN ORGANIC-SOLVENTS, Biotechnology letters, 16(10), 1994, pp. 1069-1074
Citations number
22
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
16
Issue
10
Year of publication
1994
Pages
1069 - 1074
Database
ISI
SICI code
0141-5492(1994)16:10<1069:MOLFPS>2.0.ZU;2-H
Abstract
Lipase from Pseudomonas sp. was modified with the new amphipathic poly mer poly(acryloymorpholine) - succinimidyl ester. The lipase derivativ e, which carried about 4.5 polymer chains per enzyme molecule, retaine d 54% of the original activity and showed a single symmetrical peak in gel filtration chromatography. The derivative displayed high transest erification activity in several chlorinated solvents. Compared to meth oxypoly(ethylen glycol)-modified lipase, it showed lower solubility bu t better catalytic properties in organic media.