A Packed-bubble tower was used as absorber to treat 1000m(3) biogas pe
r day. A Biofilm reactor was used as the regeneration equipment in whi
ch Thiobacillus(s ferrooxidans was employed to oxidize Fe(II)) to Fe(I
II). More than 95% H2S removal could be obtained when the inlet H2S co
ncentration of the biogas ranged from 1.0 to 2.5 g/m3 and the maximum
Fe(II) oxidation rate of bioreactor was 1170.87mg/L.h.