STOPPED LOWER BUFFER FLOW ELECTROFRACTIONATION - SIMPLE ELECTROFRACTIONATION FOR COMPLEX PROTEIN MIXTURE

The standard electrofractionation technique simulated the liquid chrom atography to create a continuous elution system. Though this continuou s lower buffer flow electrofractionation system served as an excellent model for preparative electrophoresis, the restricted current flow ca used by electric resistance arising from the column holder prevented t he application to the purification of complex protein mixtures. To ove rcome the electric current problem, the stopped lower buffer flow elec trofractionation (SFE) system is designed to work without using a colu mn holder and use prestained markers as the detection tool of protein position during the electrofractionation process. Through this improve ment, the SFE system widens the availability of electrofractionation t o the purification of complex and low-concentration protein mixtures. In this report, the efficiency of this technique is shown through the partial purification of a P-32-labeled protein in the rat liver micros ome. (C) 1994 Academic Press, Inc.