THE SEQUENCE OF A 22.4-KB DNA FRAGMENT FROM THE LEFT ARM OF YEAST CHROMOSOME-II REVEALS HOMOLOGS TO BACTERIAL PROLINE SYNTHETASE AND MURINEALPHA-ADAPTIN, AS WELL AS A NEW PERMEASE AND A DNA-BINDING PROTEIN

We report the sequencing of a 22 470 bp DNA fragment from the left arm of Saccharomyces cerevisiae chromosome II. Thirteen open reading fram es longer than 300 bp provisionally called YBL0520, YBL0401 to YBL0408 and YBL0410 to YBL0413 have been detected. Five genes were previously sequenced: COR1, encoding a core protein of the mitochondrial coenzym e QH(2) cytochrome c reductase complex (Tzagaloff and Crivellone, 1986 ), PRS3, a proteasome subunit gene (Lee et al., 1992), ERD2, coding fo r a protein involved in the secretory pathway (Semeza et al., 1990), U RA7, which encodes a CTP synthetase (Ozier-Kalogeropoulos et al., 1991 ) and the gene for the ribosomal protein L16 (Pan et al., 1993). Among the others, YBL0406 shows striking homologies to FUR4 (Jund et al., 1 988) and DAL4 (Yoo et al., 1992), the uracyl and allantoin permeases; YBL0520 is a DNA-related protein, possibly involved in gene regulation ; YBL0412 shares homologies with the mouse alpha-adaptins A and C; and YBL0413 is homologous to a protein of Pseudomonas aeruginosa that is likely to be involved in proline biosynthesis. YBL0401, internal to YB L0520, is probably not expressed. The sequence has been deposited in t he EMBL data library under accession number X78217