VARIATION OF ALKALOID PRODUCTIVITY AMONG SEVERAL CLONES OF HAIRY ROOTS AND REGENERATED PLANTS OF ATROPA-BELLADONNA TRANSFORMED WITH AGROBACTERIUM-RHIZOGENES-15834

Hairy root cultures of Atropabelladonna were established by transforma tion with Agrobacterium rhizogenes 15834. Five clones of them were emp loyed to study the production of hyoscyamine, the main constituent of the plant, together with other tropane alkaloids. The growth and alkal oid production of each clone were differently affected by basal liquid culture media tested. The transgenic plants regenerated from each clo ne of the hairy roots had different phenotypes and diverse alkaloid pr oductivity both in the cultured condition and in hydroponics.