RAPID ISOLATION OF PCR-READY DNA FROM BLOOD, BONE-MARROW AND CULTURED-CELLS, BASED ON PARAMAGNETIC BEADS

PCR-based methods for the analysis of genomic DNA are becoming increas ingly common both in research and for routine purposes. A rapid, small -scale DNA isolation method is needed to take full advantage of the sp eed and automation potential of the PCR technology. We demonstrate the use of Dynabeads(R) DNA DIRECTTM, a kit for the isolation of PCR-read y genomic DNA front whole blood, bone marrow or cultured cells in less than 10 min. The method is based on adsorption to magnetic beads prio r to magnetic separation and involves no centrifugation steps or organ ic solvents. The yield and quality of the DNA is comparable to traditi onal large-scale methods. One isolation is enough for at least ten PCR s.