DIRECTED EVOLUTION OF NOVEL BIOSYNTHETIC PATHWAYS - CONSTRUCTION OF ARANDOMLY MUTATED GENE LIBRARY IN AN ESCHERICHIA-COLI PROLINE AUXOTROPH AND SELECTION FOR GROWTH ON POTENTIAL PROLINE PRECURSORS

Authors
SUTHERLAND JD WILSON EJ WRIGHT MC
Citation
Jd. Sutherland et al., DIRECTED EVOLUTION OF NOVEL BIOSYNTHETIC PATHWAYS - CONSTRUCTION OF ARANDOMLY MUTATED GENE LIBRARY IN AN ESCHERICHIA-COLI PROLINE AUXOTROPH AND SELECTION FOR GROWTH ON POTENTIAL PROLINE PRECURSORS, Bioorganic & medicinal chemistry letters, 4(21), 1994, pp. 2551-2554
Citations number
10
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
4
Issue
21
Year of publication
1994
Pages
2551 - 2554
Database
ISI
SICI code
0960-894X(1994)4:21<2551:DEONBP>2.0.ZU;2-V
Abstract
By introducing a plasmid library into a highly mutagenising Escherichi a coil proline auxotroph the evolutionary scenario of gene doubling an d mutagenesis has been mimicked allowing for evolved growth on certain potential proline precursors.