DIRECTED EVOLUTION OF NOVEL BIOSYNTHETIC PATHWAYS - OVEREXPRESSION OFLEUCINE AMINOPEPTIDASE ALLOWS AN ESCHERICHIA-COLI PROLINE AUXOTROPH TO GROW FASTER ON PROLINAMIDE

Authors
LITTLE AJ SUTHERLAND JD WILSON EJ WOODCOLLINS DAJ
Citation
Aj. Little et al., DIRECTED EVOLUTION OF NOVEL BIOSYNTHETIC PATHWAYS - OVEREXPRESSION OFLEUCINE AMINOPEPTIDASE ALLOWS AN ESCHERICHIA-COLI PROLINE AUXOTROPH TO GROW FASTER ON PROLINAMIDE, Bioorganic & medicinal chemistry letters, 4(21), 1994, pp. 2555-2558
Citations number
8
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
4
Issue
21
Year of publication
1994
Pages
2555 - 2558
Database
ISI
SICI code
0960-894X(1994)4:21<2555:DEONBP>2.0.ZU;2-R
Abstract
Selection of plasmids which confer a rapid growth phenotype on prolina mide from a library maintained in an Escherichia coli proline auxotrop h reveals aminopeptidase I to be responsible for the growth; the enzym e has been overproduced, purified to homogeneity, characterised and cr ystallised in a form suitable for structure determination.