DIRECT IMAGING IN A WATER LAYER OF HUMAN-CHROMOSOME FIBERS COMPOSED OF NUCLEOSOMES AND THEIR HIGHER-ORDER STRUCTURES BY LASER-PLASMA X-RAY CONTACT MICROSCOPY

Authors
KINJO Y SHINOHARA K ITO A NAKANO H WATANABE M HORIIKE Y KIKUCHI Y RICHARDSON MC TANAKA KA
Citation
Y. Kinjo et al., DIRECT IMAGING IN A WATER LAYER OF HUMAN-CHROMOSOME FIBERS COMPOSED OF NUCLEOSOMES AND THEIR HIGHER-ORDER STRUCTURES BY LASER-PLASMA X-RAY CONTACT MICROSCOPY, Journal of Microscopy, 176, 1994, pp. 63-74
Citations number
14
Categorie Soggetti
Microscopy
Journal title
Journal of Microscopy → ACNP
ISSN journal
00222720
Volume
176
Year of publication
1994
Part
1
Pages
63 - 74
Database
ISI
SICI code
0022-2720(1994)176:<63:DIIAWL>2.0.ZU;2-Q
Abstract
X-ray contact microscopy with a 300-ps-duration laser-plasma X-ray sou rce has been used to image hydrated human chromosomes. Clearly imaged are individual nucleosomes and their higher-order particles (superbead s), elementary chromatin fibrils c. 30nm in diameter and their higher- order fibres of various sizes up to c. 120nm in diameter. The results demonstrate that X-ray microscopy is now capable of opening a new path of investigation into the detailed structures of hydrated chromosome fibres in their natural state.