GENETIC FINGERPRINTING OF SUNFLOWER LINES AND F1 HYBRIDS USING ISOZYMES, SIMPLE AND REPETITIVE SEQUENCES AS HYBRIDIZATION PROBES, AND RANDOM PRIMERS FOR PCR

Authors
MOSGES G FRIEDT W
Citation
G. Mosges et W. Friedt, GENETIC FINGERPRINTING OF SUNFLOWER LINES AND F1 HYBRIDS USING ISOZYMES, SIMPLE AND REPETITIVE SEQUENCES AS HYBRIDIZATION PROBES, AND RANDOM PRIMERS FOR PCR, Plant breeding, 113(2), 1994, pp. 114-124
Citations number
40
Categorie Soggetti
Plant Sciences",Agriculture
Journal title
Plant breeding → ACNP
ISSN journal
01799541
Volume
113
Issue
2
Year of publication
1994
Pages
114 - 124
Database
ISI
SICI code
0179-9541(1994)113:2<114:GFOSLA>2.0.ZU;2-L
Abstract
Three different marker systems which have been widely described in the characterization of genotypes were compared. Sunflower lines and hybr ids were analysed by three isozymes, oligonucleotides (GACA)(4), (GATA )(4) and (GGAT)(4) as hybridization probes, and 70 primers for PCR ana lysis. Polymorphisms were visible with each type of marker. However, t he repeated probes, especially (GATA)(4), revealed the highest degree of variability and seem to be most appropriate for the generation of g enotype-specific 'fingerprints.'