THE EFFECTS OF PH, IONIC-STRENGTH AND POLYVALENT IONS ON THE CELL-SURFACE HYDROPHOBICITY OF ESCHERICHIA-COLI EVALUATED BY THE BATH AND HIC METHODS

Authors
BUNT CR JONES DS TUCKER IG
Citation
Cr. Bunt et al., THE EFFECTS OF PH, IONIC-STRENGTH AND POLYVALENT IONS ON THE CELL-SURFACE HYDROPHOBICITY OF ESCHERICHIA-COLI EVALUATED BY THE BATH AND HIC METHODS, International journal of pharmaceutics, 113(2), 1995, pp. 257-261
Citations number
33
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
International journal of pharmaceutics → ACNP
ISSN journal
03785173
Volume
113
Issue
2
Year of publication
1995
Pages
257 - 261
Database
ISI
SICI code
0378-5173(1995)113:2<257:TEOPIA>2.0.ZU;2-C
Abstract
The effects of pH, ionic strength and polyvalent ions upon the subsequ ent adherence of E. coli to octyl-sepharose in hydrophobic interaction chromatography (HIC), and the effect of polyvalent ions on adherence to dichloromethane in the bacterial adherence to hydrocarbons (BATH) t ests were investigated. Ionic strength, pH and polyvalent ions were fo und to influence adhesion. Lowering pH and increasing ionic strength i ncreased adherence of E. coli to octyl-Sepharose and dichloromethane. However, polyvalent ions increased adherence to octyl-Sepharose while reducing adherence to dichloromethane, indicating the possibility of d ifferent mechanisms of interaction between E. coli and these substrate s.