EFFECT OF SODIUM DODECYL-SULFATE ON LIPASE OF CANDIDA-LIPOLYTICA

The effects of sodium dodecyl sulfate on extracellular lipase produced by Candida lipolytica have been studied. The microorganism was grown in culture medium containing different sodium dodecyl sulfate concentr ations added to the culture at different intervals of growth. The extr acellular lipase activity was not detected when the treated culture su pernatants were directly tested in Yeast Mold Agar-Triolein-Rhodamine plates, regardless of surfactant addition time and concentrations. How ever, after ammonium sulfate precipitation and dialysis, the extracell ular lipase activity could be recovered. Therefore, the surfactant, un der the experimental conditions used here, does not seem to be able to inhibit lipase production, but it does inhibit the enzyme activity be cause of its presence in the mixture of the reaction.