A P1-BASED PHYSICAL MAP OF THE REGION FROM D17S776 TO D17S78 CONTAINING THE BREAST-CANCER SUSCEPTIBILITY GENE BRCA1

Authors
NEUHAUSEN SL SWENSEN J MIKI Y LIU Q TAVTIGIAN S SHATTUCKEIDENS D KAMB A HOBBS MR GINGRICH J SHIZUYA H KIM UJ COCHRAN C FUTREAL PA WISEMAN RW LYNCH HT TONIN P NAROD S CANNONALBRIGHT L SKOLNICK MH GOLDGAR DE
Citation
Sl. Neuhausen et al., A P1-BASED PHYSICAL MAP OF THE REGION FROM D17S776 TO D17S78 CONTAINING THE BREAST-CANCER SUSCEPTIBILITY GENE BRCA1, Human molecular genetics, 3(11), 1994, pp. 1919-1926
Citations number
34
Categorie Soggetti
Genetics & Heredity",Biology
Journal title
Human molecular genetics → ACNP
ISSN journal
09646906
Volume
3
Issue
11
Year of publication
1994
Pages
1919 - 1926
Database
ISI
SICI code
0964-6906(1994)3:11<1919:APPMOT>2.0.ZU;2-T
Abstract
BRCA1, a breast and ovarian cancer susceptibility locus, has been isol ated and maps to 17q21. A physical map of the BRCA1 region which exten ded from the proximal boundary at D17S776 to the distal boundary at D1 7S78 was constructed and consists of 51 sequence tagged sites (STSs) f rom P1 and YAC ends, nine new short-tandem repeat (STR) polymorphic ma rkers, and eight identified genes. The contig, which spans the estimat ed 2.3 Mb region, contains 29 P1s, 11 YACs, two BACs, and one cosmid. Based on key recombinants in two linked families, BRCA1 was further lo calized to a region bounded by D17S1321 on the proximal side and D17S1 325 on the distal side. Within this estimated 600 kb region, the conti g was composed completely of P1s and BACs ordered by STS-content mappi ng and confirmed by DNA restriction fragment fingerprinting.