SUGAR-CONTAINING LIPIDS PREPARED BY USING A LIPOPHILIC RADICAL INITIATOR - INTERFACIAL RECOGNITION BY LECTIN AS STUDIED BY USING THE MULTIPLE INTERNAL-REFLECTION FLUORESCENCE METHOD

A glucose-containing monomer (2-methacryloylolxyethyl alpha-D-glucopyr anoside, MEG) was polymerized by using a lipophilic radical initiator. The amphiphile obtained formed a stable liposome by mixing with L-alp ha-dipalmitoylphosphatidylcholine (DPPC) or ns,trans-2,4-dioctadecadie noyl)phosphatidylcholine (DDPC, polymerized by UV irradiation after pr eparation of the liposome), and the interaction of sugar moieties of t he amphiphile with a lectin at liquid-lipid and solid-lipid interfaces was investigated by the turbidimetry and the multiple internal reflec tion fluorescence method, respectively. The rate of binding of the sug ar-containing liposome to the surface modified with the lectin was sma ller than the theoretical value for a simple collision and strongly de pendent on both weight percent of the sugar lipid in the liposome and the degree of polymerization of MEG.