DIFFERENTIAL EXPRESSION OF MARCKS AND OTHER CALMODULIN-BINDING PROTEIN-KINASE-C SUBSTRATES IN CULTURED NEUROBLASTOMA AND GLIOMA-CELLS

Expression of the protein kinase C substrate MARCKS and other heat-sta ble myristoylated proteins have been studied in four cultured neural c ell lines. Amounts of MARCKS protein, measured by [H-3]myristate label ing and western blotting, were severalfold higher in rat C6 glioma and human HTB-11 (SK-N-SH) neuroblastoma cells than in HTB-10 (SK-N-MC) o r mouse N1E-115 neuroblastoma cells. Higher levels of MARCKS mRNA were also detected in the former cell lines by S1 nuclease protection assa y. At least two additional H-3-myristoylated proteins of 50 and 40-45 kDa were observed in cell extracts heated to >80 degrees C or treated with perchloric acid. The 50-kDa protein, which bound to calmodulin in the presence of Ca2+, was more prominent in cells (N1E-115 and HTB-10 ) with less MARCKS, whereas neuromodulin (GAP-43) was detected in N1E- 115 and HTB-11 cells only. Heating resulted in a fourfold increase in the detection of MARCKS by western blotting; this was not paralleled b y a similar increase in [H-3]myristate-labeled MARCKS and may be due t o a conformational change affecting the C-terminal epitope or enhanced retention of the protein on nitrocellulose. Addition of beta-12-O-tet radecanoylphorbol 13-acetate resulted in three- to fourfold increased phosphorylation of MARCKS in HTB-11 cells, with little increase noted in HTB-10 cells. These results indicate that MARCKS, neuromodulin, and other calmodulin-binding protein kinase C substrates exhibit distinct levels of expression in cultured neurotumor cell lines. Of these prot eins, only MARCKS appears to be correlated with phorbol ester stimulat ion of phosphatidylcholine turnover in these cells.