STABILIZATION AND COMPETITIVE-INHIBITION OF BETA-CAROTENE 15,15'-DIOXYGENASE BY CAROTENOIDS

Authors
ERSHOV YV BYKHOVSKY VY DMITROVSKII AA
Citation
Yv. Ershov et al., STABILIZATION AND COMPETITIVE-INHIBITION OF BETA-CAROTENE 15,15'-DIOXYGENASE BY CAROTENOIDS, Biochemistry and molecular biology international, 34(4), 1994, pp. 755-763
Citations number
19
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
34
Issue
4
Year of publication
1994
Pages
755 - 763
Database
ISI
SICI code
1039-9712(1994)34:4<755:SACOB1>2.0.ZU;2-5
Abstract
Carotenoids stabilize beta-carotene 15,15'-dioxygenase (CDO) during th e isolation of the enzyme from the rabbit small intestinal mucosa usin g a thiol protector and protease inhibitors and in the course of the e nzyme purification. The inhibition of CDO by lycopene, lutein and asta xanthin is competitive. Carotenoids partly protect CDO from the action of both iodacetate and trypsin due to enzyme-pseudosubstrate interact ion.