ASSIGNMENT OF THE HUMAN TYPE-I IMP DEHYDROGENASE GENE (IMPDH1) TO CHROMOSOME 7Q31.3-Q32

Two phage lambda clones that contain the 5' portion of the human type I inosine 5'-monophosphate dehydrogenase (IMPDH, EC 1.1.1.205) gene we re isolated. Both polymerase chain reaction analysis of a panel of hum an-mouse and human-hamster cell somatic hybrids using primers specific for the type I IMPDH gene and fluorescence in situ hybridization with metaphase human chromosomes using type I IMPDH genomic DNA as probes indicate that the type I IMPDH gene (symbol IMPDH1) is located on chro mosome 7. Sequential GTG-banding was performed to assign the band loca tion of the type I IMPDH gene to chromosome 7q31.3-q32. (C) 1994 Acade mic Press, Inc.