DETECTION OF BETA-2 TRANSFERRIN IN OTORRHEA AND RHINORRHEA IN A ROUTINE CLINICAL LABORATORY SETTING

Citation
De. Normansell et al., DETECTION OF BETA-2 TRANSFERRIN IN OTORRHEA AND RHINORRHEA IN A ROUTINE CLINICAL LABORATORY SETTING, Clinical and diagnostic laboratory immunology, 1(1), 1994, pp. 68-70
Citations number
13
Categorie Soggetti
Immunology,"Infectious Diseases","Medical Laboratory Technology",Microbiology
ISSN journal
1071412X
Volume
1
Issue
1
Year of publication
1994
Pages
68 - 70
Database
ISI
SICI code
1071-412X(1994)1:1<68:DOBTIO>2.0.ZU;2-E
Abstract
A simple, straightforward, and rapid method for the detection of beta- 2 transferrin in otorrhea and rhinorrhea that can be used in a routine clinical laboratory is described. The beta-2 transferrin was detected by agarose gel electrophoresis of the fluid on Beckman Paragon equipm ent, followed by pressure transfer to a nitrocellulose membrane and th en incubation with enzyme-labeled antitransferrin antibody and substra te. The procedure was fast (3.5 h) and sensitive (detected as little a s 1 mu g/ml) and required only 3 mu l of fluid. Beta-2 transferrin was detected in cerebrospinal fluid diluted up to eightfold. No special t raining or expertise was needed, and all equipment and procedures used are commonly available in a routine clinical laboratory.