De. Normansell et al., DETECTION OF BETA-2 TRANSFERRIN IN OTORRHEA AND RHINORRHEA IN A ROUTINE CLINICAL LABORATORY SETTING, Clinical and diagnostic laboratory immunology, 1(1), 1994, pp. 68-70
A simple, straightforward, and rapid method for the detection of beta-
2 transferrin in otorrhea and rhinorrhea that can be used in a routine
clinical laboratory is described. The beta-2 transferrin was detected
by agarose gel electrophoresis of the fluid on Beckman Paragon equipm
ent, followed by pressure transfer to a nitrocellulose membrane and th
en incubation with enzyme-labeled antitransferrin antibody and substra
te. The procedure was fast (3.5 h) and sensitive (detected as little a
s 1 mu g/ml) and required only 3 mu l of fluid. Beta-2 transferrin was
detected in cerebrospinal fluid diluted up to eightfold. No special t
raining or expertise was needed, and all equipment and procedures used
are commonly available in a routine clinical laboratory.