Cs. Chung et al., MONOCLONAL-ANTIBODIES THAT DISTINGUISH BETWEEN ENCEPHALITOGENIC BOVINE HERPESVIRUS TYPE-1.3 AND RESPIRATORY BOVINE HERPESVIRUS TYPE-1.1, Clinical and diagnostic laboratory immunology, 1(1), 1994, pp. 83-88
Seven mouse hybridoma cell lines producing monoclonal antibodies (MAb)
against an encephalitogenic strain of bovine herpesvirus type 1.3 (BH
V-1.3) were established. The clones producing MAb were selected to be
specific for BHV-1.3 by enzyme-linked immunosorbent assay. Only L1B ne
utralized virus without complement. With the addition of complement, f
ive of the MAb neutralized BHV-1.3 but not the respiratory strain BHV-
1.1. The anti-BHV-1.3-specific MAb Q10B, L6G, and L1B precipitated gly
coproteins from BHV-1.3 that were analogous to the gI, gIII, and gIV g
lycoproteins of BHV-1.1, respectively. The other four MAb precipitated
unknown proteins. None of the anti-BHV-1.3 MAb precipitated BHV-1.1 g
lycoproteins. The majority of the anti-BHV-1.3 MAb did not react with
BHV-1.1 by immunoblotting, but O7E (unknown protein pattern by radioim
munoprecipitation) was reactive with five proteins (M(r)s of 33,000, 4
3,000, 70,000, 141,000, and 190,000) of BHV-1.3 and with a different p
attern of proteins of BHV-1.1 (M(r)s of 30,000, 38,000, 83,000, and 14
4,000). Two of the MAb, L6G and O7E, conjugated with peroxidase were f
ound to be useful for detecting BHV-1.3 antigen by immunochemistry in
Formalin-fixed brain tissue from experimentally infected calves.