MONOCLONAL-ANTIBODIES THAT DISTINGUISH BETWEEN ENCEPHALITOGENIC BOVINE HERPESVIRUS TYPE-1.3 AND RESPIRATORY BOVINE HERPESVIRUS TYPE-1.1

Seven mouse hybridoma cell lines producing monoclonal antibodies (MAb) against an encephalitogenic strain of bovine herpesvirus type 1.3 (BH V-1.3) were established. The clones producing MAb were selected to be specific for BHV-1.3 by enzyme-linked immunosorbent assay. Only L1B ne utralized virus without complement. With the addition of complement, f ive of the MAb neutralized BHV-1.3 but not the respiratory strain BHV- 1.1. The anti-BHV-1.3-specific MAb Q10B, L6G, and L1B precipitated gly coproteins from BHV-1.3 that were analogous to the gI, gIII, and gIV g lycoproteins of BHV-1.1, respectively. The other four MAb precipitated unknown proteins. None of the anti-BHV-1.3 MAb precipitated BHV-1.1 g lycoproteins. The majority of the anti-BHV-1.3 MAb did not react with BHV-1.1 by immunoblotting, but O7E (unknown protein pattern by radioim munoprecipitation) was reactive with five proteins (M(r)s of 33,000, 4 3,000, 70,000, 141,000, and 190,000) of BHV-1.3 and with a different p attern of proteins of BHV-1.1 (M(r)s of 30,000, 38,000, 83,000, and 14 4,000). Two of the MAb, L6G and O7E, conjugated with peroxidase were f ound to be useful for detecting BHV-1.3 antigen by immunochemistry in Formalin-fixed brain tissue from experimentally infected calves.