PRODUCTION OF ANTISERA AGAINST THE ENTEROTOXIN OF BACTEROIDES-FRAGILIS AND THEIR USE IN A CYTOTOXICITY NEUTRALIZATION ASSAY OF HT-29 CELLS

Authors
VANTASSELL RL LYERLY DM WILKINS TD
Citation
Rl. Vantassell et al., PRODUCTION OF ANTISERA AGAINST THE ENTEROTOXIN OF BACTEROIDES-FRAGILIS AND THEIR USE IN A CYTOTOXICITY NEUTRALIZATION ASSAY OF HT-29 CELLS, Clinical and diagnostic laboratory immunology, 1(4), 1994, pp. 473-476
Citations number
18
Categorie Soggetti
Immunology,"Infectious Diseases","Medical Laboratory Technology",Microbiology
Journal title
Clinical and diagnostic laboratory immunology → ACNP
ISSN journal
1071412X
Volume
1
Issue
4
Year of publication
1994
Pages
473 - 476
Database
ISI
SICI code
1071-412X(1994)1:4<473:POAATE>2.0.ZU;2-K
Abstract
To study the enterotoxin of Bacteroides fragilis, the colon carcinoma cell line HT-29 was used in a standard cytotoxicity assay. We produced high-titer neutralizing antisera in rabbits and goats against both cr ude and purified toxin and developed a cytotoxicity neutralization ass ay for use in confirming enterotoxin activity in culture filtrates and stools. The neutralization titers of the antisera on the colon carcin oma cell line HT-29 ranged from 1,600 to 2,400. In an antibody screeni ng enzyme-linked immunosorbent assay, titers ranged from 10(4) to 10(5 ). The antisera produced against the highly purified toxin also neutra lized the enterotoxic activity of the toxin and were mono specific by im immunoelectrophoresis.