ANALYSIS OF HUMAN ANTIVIRAL CYTOTOXIC T-LYMPHOCYTE RESPONSES FOR VACCINE TRIALS USING CRYOPRESERVED MONONUCLEAR LEUKOCYTES - DEMONSTRATION OF FEASIBILITY WITH INFLUENZA VIRUS-SPECIFIC RESPONSES

The feasibility of measuring virus-specific human cytotoxic T-lymphocy te (CTL) activity by using cryopreserved mononuclear leukocytes to sup port clinical vaccine trials was addressed. Autologous fresh and cryop reserved cells from the same sample of peripheral blood were used as s ources of CTL precursors and were tested for influenza virus-specific activity. The data indicated that virus-specific CTL activity could be measured by using cryopreserved cells; this could also be done in ass ays that are designed to characterize the responsible effector cell po pulation.