Protein samples containing ionic surfactants such as sodium dodecyl su
lfate (SDS) can be analyzed by matrix-assisted laser desorption/ioniza
tion mass spectrometry, contrary to what is currently accepted, SDS at
varying concentrations was added to four polypeptide samples, As expe
cted, a decrease in the signal was observed for increasing concentrati
ons of SDS up to 0.1% (w/v), at which no signal was observed for three
of the analytes. However, a recovery of the signals of all the analyt
es was observed at concentrations of SDS above 0.3%, with useful spect
ra at concentrations as high as 10%. The SDS concentration at which si
gnal recovery starts is always the same, regardless of the type and th
e concentration of the analyte. Another two surfactants holamidopropyl
)dimethylammonio]-1-propanesulfonate (CHAPS) and octylphenolpoly(ethyl
ene glycol ether) 10 (Triton X-100) were also tested, The trend for si
gnal variation with CHAPS addition is similar to that for SDS addition
, but for Triton X-100, the signal always diminishes with increasing s
urfactant concentration, For protein samples containing surfactants wi
th anionic groups, addition of more surfactant allows the acquisition
of useful mass spectrometric data.