CAPILLARY ELECTROPHORESIS OF SUPERCOILED AND LINEAR DNA IN DILUTE HYDROXYETHYL CELLULOSE SOLUTION

Authors
HAMMOND RW OANA H SCHWINEFUS JJ BONADIO J LEVY RJ MORRIS MD
Citation
Rw. Hammond et al., CAPILLARY ELECTROPHORESIS OF SUPERCOILED AND LINEAR DNA IN DILUTE HYDROXYETHYL CELLULOSE SOLUTION, Analytical chemistry, 69(6), 1997, pp. 1192-1196
Citations number
22
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry → ACNP
ISSN journal
00032700
Volume
69
Issue
6
Year of publication
1997
Pages
1192 - 1196
Database
ISI
SICI code
0003-2700(1997)69:6<1192:CEOSAL>2.0.ZU;2-5
Abstract
Capillary electrophoresis in dilute hydroxyethyl cellulose is shown to separate supercoiled DNA in the size range 2000-16 000 base pairs. Th e plasmids migrate more slowly than linear ds-DNA of the same sizes. P lasmid bandwidths are larger than observed for ds-DNA, allowing identi fication of the type of DNA by bandwidth. The differing dependence of mobility on chain length can be explained by assuming that a plasmid m igrates as an elastic rod, while ds-DNA migrates as a wormlike chain.